Research Assistant 2 job – Case Western Reserve University – Cleveland, OH


Working under general supervision, plan and carry out research project in accordance with general plans. The objective of this laboratory is to understand the role of cholesterol-related proteins in retinal structure and function. The position’s role is to characterize the retinal phenotype of genetically modified mice by in vivo and in vitro techniques. This position works with animals.


Plan and carry out in vivo retinal imaging by SD-OCT and fluorescein angiography. 35%

Conduct a series of in vitro research including but not limited to Western blot, qRT-PCR, histo- and immunohistochemistry to supply scientific information as part of a larger project. 30%

Conduct analysis of experiments. 10%

Maintain experimental records and record results of conducted experiments. 10%


Train new staff; direct the work of students. 5%

Maintain test equipment utilized in experiments. 3%

Assist in developing improved techniques, projection methods or procedures. 2.5%

Oversee mouse inventory. 2.5%

Perform other duties as assigned. 2%


Department: Daily contact with supervisor to discuss research

University: Occasional contact with other departments to share information.

External: Limited or no contact with vendors to exchange information.

Students: Daily contact with student employees.


This position has no direct supervision of staff employees. Train new staff; direct the work of students.


Education and Experience: Bachelor’s degree and 1 to 3 years of related experience or an or Associate’s degree in an approved biotechnology program and 2 to 4 years of experience.


Ability to work with and sacrifice mice, no allergies to animals.

Has knowledge of the eye anatomy and function, understanding of the principles of SD-OCT and fluorescein angiography, ability to perform Western blot, qRT-PCT, histo- and immunohistochemistry.

Rely on instructions and pre-established guidelines to perform the functions of the job.

Ability to operate laboratory equipment.

Must be a highly responsible, careful, and motivated individual with good organizational, lab maintenance and planning skills, ability to manage time effectively, ability to coordinate work on several ongoing projects.

Fluent in written and spoken English and be flexible for some weekend schedules.

Must demonstrate compliance with CWRU animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.

Must demonstrate compassion for animals within CWRU facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.

Previous experience working with animals preferred.


General laboratory environment. The employee may have exposure to hazards – animals, bloodborne pathogens, chemicals, FBI check, and laser.

Case’s animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the “Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals.” This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

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